Article
Overexpression, purification and characterization of the Escherichia coli MelR transcription activator protein.
The Biochemical journal - 15 Oct 1992
Caswell R, Williams J, Lyddiatt A, Busby S
Abstract excerpt
The gene encoding Escherichia coli MelR protein has been cloned in the expression vector pJLA502. MelR has been overexpressed, substantially purified and shown to bind to DNA fragments carrying the melAB promoter. A truncated version of the melR gene, encoding the C-terminal half of MelR, was also cloned into pJLA502; the protein product of this truncated gene binds to the melAB promoter but was not overproduced....
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Cloning, Molecular
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression
- Molecular Sequence Data
- Mutation