Article
The development of a FACS-based strategy for the isolation of Shigella flexneri mutants that are deficient in intercellular spread.
Molecular microbiology - 1 Mar 2000
Rathman M, Jouirhi N, Allaoui A, Sansonetti P, Parsot C, Tran Van Nhieu G
Abstract excerpt
In the disease course of bacillary dysentery, pathogenic Shigella flexneri invade colonic epithelial cells and spread both within and between host cells. The ability to spread intercellularly allows the organism to infect an entire epithelial layer without significant contact with the extracellular milieu. Using fluorescence activated cell sorter (FACS)-based technology, we developed a rapid and powerful...
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