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Article

Analysis of Tlr4-mediated LPS signal transduction in macrophages by mutational modification of the receptor.

Blood cells, molecules & diseases - 1 Jan 2000

Du X, Poltorak A, Silva M, Beutler B

Abstract excerpt

In mouse macrophages (RAW 264.7 cells), toll-like receptor 4 (Tlr4) is a limiting factor in lipopolysaccharide (LPS) signal transduction. The expression of only 1-2 x 10(4) copies of recombinant Tlr4 per cell enhances sensitivity to LPS, shifting the EC50 by 30-fold to the left. Expression of the Tlr4(Lps-d) isoform of Tlr4 (found in C3H/HeJ mice) shifts the EC50 2600-fold to the right, essentially abolishing LPS...

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Source

Identifiers

PMID: 10660480
DOI: 10.1006/bcmd.1999.0262

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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