Article
Characterization of a double gene disruption in the LYS2 locus of the pathogenic yeast, Candida albicans.
Medical mycology - 1 Dec 1999
Bhattacherjee V, Bhattacharjee J K
Abstract excerpt
The 4.2 kbp LYS2 gene of Candida albicans encodes the 150 kDa subunit of the 180 kDa heterodimeric enzyme alpha-aminoadipate reductase. To facilitate structural and functional studies of the LYS2 gene, in this investigation both alleles were sequentially disrupted in C. albicans CAI4. The disruptions were performed using a targeting vector that contained a 2.2 kbp portion of LYS2 from which a 600-bp fragment had...
Topics
- Aldehyde Oxidoreductases
- Candida albicans
- Chromosome Mapping
- Genes, Fungal
- L-Aminoadipate-Semialdehyde Dehydrogenase
- Mutation