Isolation and characterization of kar2-404 mutation in Saccharomyces cerevisiae.

doi:10.1271/bbb.61.1172

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Isolation and characterization of kar2-404 mutation in Saccharomyces cerevisiae.

Bioscience, biotechnology, and biochemistry - 1 Jul 1997

Kawamura-Watabe A, Tokunaga M

Abstract excerpt

We have devised a direct screening method to isolate mutations in the KAR2 gene, and have isolated a BiP/KAR2 mutant, kar2-404, from Saccharomyces cerevisiae as a small halo-forming mutant of secreted mouse alpha-amylase. The mutation site was identified as a point mutation at t1337 to c1337 resulting in the Ile-404Thr mutation of mature Kar2-404p, located at the most NH2-terminal first beta-sheet structure (beta...

Topics

Amino Acid Sequence
Animals
Binding Sites
Culture Media
Fungal Proteins
HSP70 Heat-Shock Proteins
Mice
Molecular Sequence Data
Mutation
Plasmids
Promoter Regions, Genetic
Protein Engineering
Protein Precursors
Recombinant Fusion Proteins
Saccharomyces cerevisiae
alpha-Amylases

Source

Open in PubMed

Identifiers

PMID: 9255982
DOI: 10.1271/bbb.61.1172

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics