Article
Nonrandomly-associated forward mutation and mitotic recombination yield yeast diploids homozygous for recessive mutations.
Current genetics - 1 Oct 1994
Esposito M S, Ramirez R M, Bruschi C V
Abstract excerpt
We have employed the analysis of spontaneous forward mutations that confer the ability to utilize L-alpha-aminoadipate as a nitrogen source (alpha-Aa+) to discern the events that contribute to mitotic segregation of spontaneous recessive mutations by diploid cells. alpha-Aa- diploid cells yield alpha-Aa+ mutants at a rate of 7.8 +/- 3.6 x 10(-9). As in haploid strains, approximately 97% (30/31) of alpha-Aa+...
Topics
- Diploidy
- Genes, Fungal
- Genes, Recessive
- Haploidy
- Homozygote
- Mitosis
- Mutation
- Recombination, Genetic
- Saccharomyces cerevisiae