Article
[Development of a system of intragenic mapping for molecular genetic analysis of mutations in the gene LYS2 of Saccharomyces yeasts].
Genetika - 1 Jul 1990
Noskov V N, Tarutina M G, Pavlov Iu I, Kulikov V N, Trofimova M V, Gorbacheva A V, Chernov Iu O, Sasnauskas K V, Neĭstat M A, Tolstorukov I I
Abstract excerpt
The collection of overlapping lys2 deletions (five in the chromosomal and seven in the plasmid LYS2 gene) is constructed in this work. The deletions overlap the whole coding region of the gene and provide the system for intragenic recombinational mapping of lys2 mutations in one of 14 controlled...
Topics
- Alleles
- Chromosome Deletion
- Chromosomes, Fungal
- Genes, Fungal
- Genetic Engineering
- Mutation
- Plasmids
- Restriction Mapping
- Saccharomyces cerevisiae