High-level rearrangement and transcription of yeast artificial chromosome-based mouse Ig kappa transgenes containing distal regions of the contig.

doi:10.4049/jimmunol.164.2.812

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Article

High-level rearrangement and transcription of yeast artificial chromosome-based mouse Ig kappa transgenes containing distal regions of the contig.

Journal of immunology (Baltimore, Md. : 1950) - 15 Jan 2000

Li S, Hammer R E, George-Raizen J B, Meyers K C, Garrard W T

Abstract excerpt

The mouse Ig kappa L chain gene locus has been extensively studied, but to date high-level expression of germline transgenes has not been achieved. Reasoning that each end of the locus may contain regulatory elements because these regions are not deleted upon V kappa-J kappa joining, we used yeast artificial chromosome-based techniques to fuse distal regions of the contig to create transgene miniloci. The largest...

Topics

Alleles
Animals
B-Lymphocytes
Cell Differentiation
Chromosomes, Artificial, Yeast
Contig Mapping
Crosses, Genetic
Gene Dosage
Gene Rearrangement, B-Lymphocyte, Light Chain
Genes, Immunoglobulin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Markers

Germ Cells

Immunoglobulin Joining Region

Immunoglobulin Variable Region

Immunoglobulin kappa-Chains

Mice

Mice, Transgenic

Multigene Family

Source

Open in PubMed

Identifiers

PMID: 10623827
DOI: 10.4049/jimmunol.164.2.812

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