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Article

Role of adenine functional groups in the recognition of the 3'-splice-site AG during the second step of pre-mRNA splicing.

Proceedings of the National Academy of Sciences of the United States of America - 4 Jan 2000

Gaur R K, Beigelman L, Haeberli P, Maniatis T

Abstract excerpt

The AG dinucleotide at the 3' splice sites of metazoan nuclear pre-mRNAs plays a critical role in catalytic step II of the splicing reaction. Previous studies have shown that replacement of the guanine by adenine in the AG (AG --> GG) inhibits this step. We find that the second step was even more severely inhibited by cytosine (AG --> CG) or uracil (AG --> UG) substitutions at this position. By contrast, a...

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Identifiers

PMID: 10618380
DOI: 10.1073/pnas.97.1.115
PMCID: PMC26625

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Source receipt: pubmed, retrieved 7 Sept 2026.

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