Article
Role of adenine functional groups in the recognition of the 3'-splice-site AG during the second step of pre-mRNA splicing.
Proceedings of the National Academy of Sciences of the United States of America - 4 Jan 2000
Gaur R K, Beigelman L, Haeberli P, Maniatis T
Abstract excerpt
The AG dinucleotide at the 3' splice sites of metazoan nuclear pre-mRNAs plays a critical role in catalytic step II of the splicing reaction. Previous studies have shown that replacement of the guanine by adenine in the AG (AG --> GG) inhibits this step. We find that the second step was even more severely inhibited by cytosine (AG --> CG) or uracil (AG --> UG) substitutions at this position. By contrast, a...
Topics
- Adenine
- Base Sequence
- Hydrogen Bonding
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Plasmids
- RNA Precursors
- RNA Splicing
- Substrate Specificity
- Time Factors