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Article

Isoguanosine substitution of conserved adenosines in the hammerhead ribozyme.

Biochemistry - 11 Oct 1994

Ng M M, Benseler F, Tuschl T, Eckstein F

Abstract excerpt

Isoguanosine has been incorporated into a 34-mer hammerhead ribozyme by the solid-phase phosphoramidite method, using an acetamidine base protecting group. The activity of the hammerhead ribozyme when singly mutated to isoguanosine at the adenosine positions 6, 9, and 13 was 1-2-fold less than the wild-type activity. Mutations to 2-aminopurine ribonucleoside at positions 9 and 13 were 5-fold reduced in activity,...

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Identifiers

PMID: 7918433
DOI: 10.1021/bi00206a015

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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