Isolation of Bacteroides fragilis mutants with in vivo growth defects by using Tn4400', a modified Tn4400 transposition system, and a new screening method.

Abstract excerpt

A modified version of the Bacteroides fragilis transposon Tn4400, designated Tn4400', enabling rapid isolation and analysis of B. fragilis mutants has been constructed. To identify potential virulence factors, Tn4400'-generated mutants were screened by a new method; this resulted in the isolation of 21 mutant strains with impaired growth characteristics on tissue culture monolayers but normal growth in rich...

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