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Article

Mutation of aspartic acid residues in the fructosyltransferase of Streptococcus salivarius ATCC 25975.

The Biochemical journal - 15 Nov 1999

Song D D, Jacques N A

Abstract excerpt

The site-directed mutated fructosyltransferases (Ftfs) of Streptococcus salivarius ATCC 25975, D312E, D312S, D312N and D312K were all active at 37 degrees C, indicating that Asp-312 present in the 'sucrose box' was not the nucleophilic Asp residue responsible for the formation of a covalent fructosyl-enzyme intermediate required for enzyme activity. Analysis of the kinetic constants of the purified mutated forms...

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Identifiers

PMID: 10548559
DOI: 10.1042/0264-6021:3440259
PMCID: PMC1220639

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