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Article

Identification of Functionally Important Amino Acid Residues in Zymomonas mobilis Levansucrase

1 Oct 2002

Abstract excerpt

The catalytic residues of levansucrase (sucrose:2,6-beta-D-fructan 6-beta-D-fructosyltransferase, EC 2.4.1.10) from Zymomonas mobilis were analyzed by random mutation and site-directed mutagenesis. We found that substitution of Glu278 with Asp and His reduced the k(cat) for sucrose hydrolysis 30- and 210-fold, respectively, strongly suggesting Glu278 plays a key role in catalyzing this reaction. Given the...

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PMID: 12359071
DOI: 10.1093/oxfordjournals.jbchem.a003258

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