Article
Identification of essential amino acids in the Streptococcus mutans glucosyltransferases.
Journal of bacteriology - 1 Jun 1997
Tsumori H, Minami T, Kuramitsu H K
Abstract excerpt
A comparison of the amino acid sequences of the glucosyltransferases (GTFs) of mutans streptococci with those from the alpha-amylase family of enzymes revealed a number of conserved amino acid positions which have been implicated as essential in catalysis. Utilizing a site-directed mutagenesis approach with the GTF-I enzyme of Streptococcus mutans GS-5, we identified three of these conserved amino acid positions,...
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