Article
Mutation of UDP-glucose binding motif residues lead to increased affinity for ADP-glucose in sugarcane sucrose phosphate synthase.
Molecular biology reports - 1 Feb 2021
Kurniah Nuriyah Inda, Sawitri Widhi Dyah, Rohman Muhammad Saifur, Nugraha Yudhi, Hase Toshiharu, Sugiharto Bambang
Abstract excerpt
Plant sucrose-phosphate synthase (SPS) contains a glycosyltransferase domain, which specifically catalyzes reactions with the nucleotide sugar uridine diphosphate glucose (UDP-G) as a donor substrate. Unlike plant SPS, bacterial SPS is predicted to bind other nucleotide sugars, such as adenosine diphosphate glucose (ADP-G). This study aimed to identify the UDP-G binding site of sugarcane (Saccharum officinarum)...
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