Article
Structure-function relationships in glucoamylases encoded by variant Saccharomycopsis fibuligera genes.
European journal of biochemistry - 1 Sept 1999
Solovicová A, Christensen T, Hostinová E, Gasperík J, Sevcĭk J, Svensson B
Abstract excerpt
The mutation Gly467-->Ser in Glu glucoamylase was designed to investigate differences between two highly homologous wild-type Saccharomycopsis fibuligera Gla and Glu glucoamylases. Gly467, localized in the conserved active site region, S5, is replaced by Ser in the Gla glucoamylase. These amino acid residues are the only two known to occupy this position in the elucidated glucoamylase sequences. The data from the...
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