Article
Tagging chromatin with retrotransposons: target specificity of the Saccharomyces Ty5 retrotransposon changes with the chromosomal localization of Sir3p and Sir4p.
Genes & development - 15 Oct 1999
Zhu Y, Zou S, Wright D A, Voytas D F
Abstract excerpt
Retrotransposon and retroviral insertions are not randomly distributed on chromosomes, suggesting that retroelements actively select integration sites. This is the case for the yeast Ty5 retrotransposons, which preferentially integrate into domains of silent chromatin at the HM loci and telomeres. Here we demonstrate that loss of Sir3p or Sir4p-components of silent chromatin-causes a greater than ninefold...
Topics
- Alleles
- Base Sequence
- Chromatin
- Chromosome Mapping
- Chromosomes, Fungal
- DNA Primers
- DNA, Complementary
- DNA, Fungal
- DNA, Ribosomal
- Fungal Proteins
- Gene Deletion
- Genes, Fungal
- Molecular Sequence Data
- Polymerase Chain Reaction