Article
Purification, solution properties and crystallization of SIV integrase containing a continuous core and C-terminal domain.
Acta crystallographica. Section D, Biological crystallography - 1 Nov 1999
Li Y, Yan Y, Zugay-Murphy J, Xu B, Cole J L, Witmer M, Felock P, Wolfe A, Hazuda D, Sardana M K, Chen Z, Kuo L C, Sardana V V
Abstract excerpt
The C-terminal two-thirds segment of integrase derived from the simian immunodeficiency virus has been cloned, expressed in Escherichia coli, and purified to greater than 95% homogeneity. The protein encompasses amino-acid residues 50-293 and contains a F185H substitution to enhance solubility. In dilute solutions at concentrations below 1 mg ml(-1), the enzyme is predominantly dimeric. At the higher...
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