Article
Characterization of feline immunodeficiency virus integrase and analysis of functional domains.
Virology - 31 Mar 1997
Shibagaki Y, Holmes M L, Appa R S, Chow S A
Abstract excerpt
The wild-type and mutant derivatives of the integrase protein of feline immunodeficiency virus (FIV) were cloned and expressed in Escherichia coli. The purified proteins were examined using various model DNA substrates for their catalytic activities: 3'-end processing, 3'-end joining, and disintegration. The reactions required the presence of either Mn2+ or Mg2+ as a divalent cation. The N-terminal and...
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