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Article

Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.

BMC biochemistry - 10 Oct 2008

Portakal Oytun, Doğan Pakize

Abstract excerpt

BACKGROUND: recD, located between recB and argA, encodes the smallest polypeptide (60 kDa) of the heterotrimeric enzyme RecBCD in Escherichia coli. RecD is a 5'-3' helicase and is required for the nuclease activity of RecBCD and for tight binding to dsDNA ends. Here, we have tested the hypothesis that RecD regulates the structure and activities of RecBCD, including RecA loading. RESULTS: To characterize its...

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Identifiers

PMID: 18847457
DOI: 10.1186/1471-2091-9-27
PMCID: PMC2586629

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of recB-recD genetic fusion and functional analysis of RecBDC fusion enzyme in Escherichia coli.PMID 18847457Read ↗

A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme.Semantic proximity Read ↗

Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme.Semantic proximity Read ↗

A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme.Semantic proximity Read ↗

Chi and the RecBC D enzyme of Escherichia coli.Semantic proximity Read ↗

Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot chi in vitro: evidence for functional inactivation or loss of the RecD subunit.Semantic proximity Read ↗

RecBCD enzyme overproduction impairs DNA repair and homologous recombination in Escherichia coli.Semantic proximity Read ↗

A RecA mutant, RecA(730), suppresses the recombination deficiency of the RecBC(1004)D-chi* interaction in vitro and in vivo.Semantic proximity Read ↗

Genetic dissection of the biochemical activities of RecBCD enzyme.Semantic proximity Read ↗

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Functions of multiple exonucleases are essential for cell viability, DNA repair and homologous recombination in recD mutants of Escherichia coli.Semantic proximity Read ↗

Biochemical characterization of a mutant recBCD enzyme, the recB2109CD enzyme, which lacks chi-specific, but not non-specific, nuclease activity.Semantic proximity Read ↗

Characterization of RecA1332 in vivo and in vitro. A role for alpha-helix E as a liaison between the subunit-subunit interface and the DNA and ATP binding domains of RecA protein.Semantic proximity Read ↗

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