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Article

Single-step PCR amplification and enzyme restriction analysis of the entire Helicobacter pylori cytotoxin vacA gene for genetic variability studies.

FEMS microbiology letters - 1 Sept 1999

Perales G, Sanchez J, Mohar A, Lara-Lemus R, Hernández A, Herrera-Goepfert R, Barrios-Jacobo I, Ayala G

Abstract excerpt

To monitor changes along the entire Helicobacter pylori vac A gene we carried out full-length single-step PCR amplification in 21 gastritis and gastric cancer isolates. HindIII restriction analysis led us to detect a > 400-bp internal insertion in vacA subsequently shown to be a direct 451-bp gene duplication. We found HindIII profiles for 16 genes that allowed their grouping into two restriction patterns that...

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Identifiers

PMID: 10483723
DOI: 10.1111/j.1574-6968.1999.tb13759.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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