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Article

A novel ELISA-based primer extension assay for the detection of the factor V Leiden mutation.

British journal of haematology - 1 Aug 1999

Wilde J T, O'Sullivan J J, Roper J L, Aerts P, Horowitz M, Navot N

Abstract excerpt

We describe an enzyme-linked immunosorbent assay (ELISA) based primer extension method for the detection of the factor V Leiden (FVL) mutation. The wild-type nucleotide at position 1691 or the mutant nucleotide at the complementary position on the antisense strand were detected by the incorporation of biotinylated complementary bases onto fluorescein isothiocyanate (FITC) labelled mini-sequence primers with...

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Identifiers

PMID: 10460602
DOI: 10.1046/j.1365-2141.1999.01536.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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