Article
Detection of the factor V Leiden mutation by a modified photo-cross-linking oligonucleotide hybridization assay.
Clinical chemistry - 1 Feb 2004
French Cynthia, Li Conan, Strom Charles, Sun Weimin, Van Atta Reuel, Gonzalez Belen, Wood Michael
Abstract excerpt
BACKGROUND: Our previously developed assay for detection of the factor V Leiden mutation (G1691A) based on a nucleic acid photo-cross-linking technology used two allele-specific capture probes and six fluorescein-modified signal-generating reporter probes. We wished to improve the sensitivity and performance of the method. METHODS: We developed new reporter probes with approximately 10-fold more fluorescein...
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