Article
Automated detection of the factor V Leiden mutation using the LCx microparticle enzyme immunoassay.
Clinical chemistry - 1 Jan 1999
Hunault M, Marsh-Scott C, Jou C, Marshall R, Scheffel C, Fiore L D, Bauer K A, Mitchell M J
Abstract excerpt
The factor V Leiden mutation, a G-->A transition at position 1691 in exon 10 of the gene that codes for factor V, produces an Arg506Gln substitution and is the most common genetic risk factor for venous thrombosis. We have developed a rapid, sensitive, and specific method to detect the factor V L...
Topics
- Autoanalysis
- DNA Mutational Analysis
- Electrophoresis, Polyacrylamide Gel
- Factor V
- Humans
- Immunoenzyme Techniques
- Male
- Middle Aged
- Mutation
- Polymerase Chain Reaction
- Reagent Kits, Diagnostic
- Sensitivity and Specificity