Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Automated detection of the factor V Leiden mutation using the LCx microparticle enzyme immunoassay.

Clinical chemistry - 1 Jan 1999

Hunault M, Marsh-Scott C, Jou C, Marshall R, Scheffel C, Fiore L D, Bauer K A, Mitchell M J

Abstract excerpt

The factor V Leiden mutation, a G-->A transition at position 1691 in exon 10 of the gene that codes for factor V, produces an Arg506Gln substitution and is the most common genetic risk factor for venous thrombosis. We have developed a rapid, sensitive, and specific method to detect the factor V Leiden mutation in genomic DNA from whole blood by PCR amplification and microparticle enzyme immunoassay detection...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Mutational Analysis
Share to Electrophoresis, Polyacrylamide Gel

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Factor V

Share to Immunoenzyme Techniques

Share to Mutation

Share to Polymerase Chain Reaction

Share to Reagent Kits, Diagnostic

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 9895336

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Automated detection of the factor V Leiden mutation using the LCx microparticle enzyme immunoassay.PMID 9895336Read ↗

Automated detection of the G20210A prothrombin mutation using the LCx microparticle enzyme immunoassay.Semantic proximity Read ↗

Diagnostic single nucleotide polymorphism analysis of factor V Leiden and prothrombin 20210G > A. A comparison of the Nanogen Eelectronic Microarray with restriction enzyme digestion and the Roche LightCycler.Semantic proximity Read ↗

Rapid automated simultaneous screening of (G1691A) Factor V, (G20210A) prothrombin, and (C677T) methylenetetrahydrofolate reductase variants by multiplex PCR using fluorescence scanning technology.Semantic proximity Read ↗

Evaluation of the GeneXpert assay in the detection of Factor V Leiden and prothrombin 20210 in stored, previously classified samples.Semantic proximity Read ↗

Evaluation of temperature gradient capillary electrophoresis for detection of the Factor V Leiden mutation: coincident identification of a novel polymorphism in Factor V.Semantic proximity Read ↗

Determination of the factor V Leiden single-nucleotide polymorphism in a commercial clinical laboratory by use of NanoChip microelectronic array technology.Semantic proximity Read ↗

A novel ELISA-based primer extension assay for the detection of the factor V Leiden mutation.Semantic proximity Read ↗

Allelic discrimination of factor V Leiden using a 5' nuclease assay.Semantic proximity Read ↗

Rapid genotyping of factor V Leiden mutation using single-tube bidirectional allele-specific amplification and automated ultrathin-layer agarose gel electrophoresis.Semantic proximity Read ↗

A whole-blood homogeneous assay for the multiplex detection of the factor V G1691A and the prothrombin G20210A mutations.Semantic proximity Read ↗

Semi-automated detection of the factor V mutation by allele specific amplification and capillary electrophoresis.Semantic proximity Read ↗

Genotyping of factor V G1691A (Leiden) without the use of PCR by invasive cleavage of oligonucleotide probes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.