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Article

Easy assessment of ES cell clone potency for chimeric development and germ-line competency by an optimized aggregation method.

Journal of biochemical and biophysical methods - 13 May 1999

Kondoh G, Yamamoto Y, Yoshida K, Suzuki Y, Osuka S, Nakano Y, Morita T, Takeda J

Abstract excerpt

Production of germ-line competent chimeric mice from embryonic stem (ES) cells is an inevitable step in establishing gene-manipulated mouse lineages. A common method used for creating chimeric mice is the injection of ES cells into the blastocoelic cavity (blastocyst injection). The aggregation method is an alternative way to introduce ES cells to the host embryo which is less difficult than blastocyst injection....

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PMID: 10392569
DOI: 10.1016/s0165-022x(99)00008-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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