Article
Embryo culture-based generation of enhanced green fluorescent protein-transgenic mice.
Biochemical and biophysical research communications - 18 Apr 2003
Devgan Vikram, Thomas Monzy, Ullas Kolathur S, Rao Manchanahalli R S, Seshagiri Polani B
Abstract excerpt
One of the limitations of transgenesis is low efficiency. In this study, we generated transgenic mice harboring the enhanced green fluorescent protein (EGFP) gene, under the control of chicken-beta-actin promoter and cytomegalovirus enhancer, using two approaches and compared their efficiencies. One involved culture of EGFP-injected embryos developing through EGFP-expressing "green" blastocysts, followed by their...
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