Further studies on thermal treatment of two-cell stage embryos to produce complete embryonic stem-cell-derived mice by cell-aggregation methods.

Abstract excerpt

Employing aggregation techniques with two embryonic sources, one from two-cell stage embryos treated by thermal stimulation and the other from mouse embryonic stem (ES) cells that had been obtained from a feeder layer, simple and most effective methods of producing a complete generation of mice from ES cells were explored. Although thermal treatment affected embryos at various developmental stages, the embryos at...

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