Article
Improved establishment of embryonic stem (ES) cell lines from the Chinese Kunming mice by hybridization with 129 mice.
International journal of molecular sciences - 25 Feb 2014
Yu Shumin, Yan Xingrong, Liu Huanhuan, Cai Xin, Cao Suizhong, Shen Liuhong, Zuo Zhicai, Deng Junliang, Ma Xiaoping, Wang Ya, Ren Zhihua
Abstract excerpt
Chinese Kunming mice (Mus musculus Km), widely used as laboratory animals throughout China, remain very refractory for embryonic stem (ES) cell isolation. The present study was aimed to evaluate the effects of hybridization with 129/Sv mice, and culture media containing fetal bovine serum (FBS) or Knockout serum replacement (KSR) on ES cell isolation from Kunming mice. The results demonstrated that ES cells had...
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