Article
A novel ES cell line, TT2, with high germline-differentiating potency.
Analytical biochemistry - 1 Oct 1993
Yagi T, Tokunaga T, Furuta Y, Nada S, Yoshida M, Tsukada T, Saga Y, Takeda N, Ikawa Y, Aizawa S
Abstract excerpt
In producing mutant mice by gene-targeting and gene-trapping in embryonic stem (ES) cells, the efficient colonization of the mutant ES cells into germline is still a critical matter. We have established a new line of ES cells, TT2, from an F1 embryo between a C57BL/6 female and a CBA male. When t...
Topics
- Animals
- Blastocyst
- Blotting, Southern
- Cell Differentiation
- Cell Line
- Chimera
- Chromosome Mapping
- Culture Techniques
- DNA
- Embryo, Mammalian
- Female
- Genetic Techniques