Article
Molecular fingerprinting reveals non-overlapping T cell oligoclonality between an inflamed site and peripheral blood.
International immunology - 1 Apr 1999
Wedderburn L R, Maini M K, Patel A, Beverley P C, Woo P
Abstract excerpt
We have demonstrated a stable expansion of CD8+ T cells in the peripheral blood of a child with chronic arthritis. The expanded TCRBV family (TCRBV14) was enriched for CD57hiCD28- T cells. Sequencing of the TCRBV14 amplification products showed a TCR sequence which contributed 32% of the total TCR in the CD8+TCRBV14 population. Using the modified heteroduplex technique, the CD8+TCRBV14 cells showed a clonal...
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