Article
Clonal expansions of Vδ1<sup>+</sup> and Vδ2<sup>+</sup> cells increase with age and limit the repertoire of human γδ T cells
1 Aug 1994
Abstract excerpt
We have investigated the complexity of the human gamma delta T cell repertoire by means of a VJ heteroduplex analysis method. cDNA obtained from peripheral blood mononuclear cells was amplified with V delta 1-C delta or V delta 2-C delta primers. The product was denatured and renatured to allow random reannealing of the strands and the heteroduplexes carrying mismatched junctional sequences were separated from...
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