Increase in gene expression by respiratory-deficient mutation.

doi:10.1002/yea.320050204

Back to search

Article

Increase in gene expression by respiratory-deficient mutation.

Yeast (Chichester, England) - 1 Jan 2000

Kaisho Y, Yoshimura K, Nakahama K

Abstract excerpt

Respiratory-deficient mutants (rho- cells) of Saccharomyces cerevisiae produced about 10 times as much human (h-) lysozyme as did wild-type strains (rho+ cells) when the GAL10 promoter was used in an expression plasmid with the h-lysozyme gene. Introduction of intact mitochondria into the rho- ce...

Topics

Blotting, Northern
Gene Expression Regulation
Muramidase
Mutation
Plasmids
Promoter Regions, Genetic
RNA, Fungal
RNA, Messenger
Saccharomyces cerevisiae
Transformation, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.