Article
A ketolide resistance mutation in domain II of 23S rRNA reveals the proximity of hairpin 35 to the peptidyl transferase centre.
Molecular microbiology - 1 Jan 1999
Xiong L, Shah S, Mauvais P, Mankin A S
Abstract excerpt
Ketolides represent a new generation of macrolide antibiotics. In order to identify the ketolide-binding site on the ribosome, a library of Escherichia coli clones, transformed with a plasmid carrying randomly mutagenized rRNA operon, was screened for mutants exhibiting resistance to the ketolide HMR3647. Sequencing of the plasmid isolated from one of the resistant clones and fragment exchange demonstrated that a...
Topics
- Anti-Bacterial Agents
- Base Sequence
- Binding Sites
- Drug Resistance, Microbial
- Erythromycin
- Escherichia coli
- Ketolides
- Macrolides
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Peptidyl Transferases
- RNA, Ribosomal, 23S
- Ribosomes