Erythromycin binding is reduced in ribosomes with conformational alterations in the 23 S rRNA peptidyl transferase loop.

Abstract excerpt

The antibiotic erythromycin inhibits protein synthesis by binding to the 50 S ribosomal subunit, where the drug interacts with the unpaired bases 2058A and 2059A in the peptidyl transferase loop of 23 S rRNA. We used a chemical modification approach to analyse conformational changes that are induced by mutations in the peptidyl transferase loop, and to determine how these changes affect drug interaction....

Topics

• Base Sequence
• Binding Sites
• Drug Resistance, Microbial
• Erythromycin
• Escherichia coli
• Molecular Sequence Data
• Mutation
• Peptidyl Transferases
• Phenotype
• Protein Conformation
• RNA, Bacterial