Article
Design and synthesis of reagents for phage display screening of dehalogenases.
Bioorganic & medicinal chemistry letters - 18 Jan 1999
Pieters R J, Fennema M, Kellogg R M, Janssen D B
Abstract excerpt
Bifunctional molecules containing both a biotin and a substrate unit have been designed and synthesized for phage display screening of mutant libraries of haloalkane dehalogenase enzymes. The molecules were assembled using a convergent modular synthetic strategy. One molecule was synthesized to evaluate the concept of covalent capture and a second for screening of phage libraries for enantioselectivity.
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