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Article

Library-on-Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides.

Chembiochem : a European journal of chemical biology - 1 Jan 2026

Lakkanasirorat Benya, Kongkamnead Phatipon, Amornloetwattana Rawiporn, Leejareon Pansa, Uttamapinant Chayasith, Wang Wenjing

Abstract excerpt

Library-on-library (LOL) selection screens combinatorial libraries to generate new protein pairs. Previously, LOL selection has only been applied to stable protein-protein interactions. To extend LOL to transient enzyme-substrate pairs, a generalizable sequential LOL trans- and cis-labeling platform is developed, and a proof-of-concept selection is performed on Escherichia coli biotin ligase (BirA) and its...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 41517932
DOI: 10.1002/cbic.202500804
PMCID: PMC12789891

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

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