Article

Efficient long single molecule sequencing for cost effective and accurate sequencing, haplotyping, and <i>de novo</i> assembly

2018-05-17

Abstract excerpt

Obtaining accurate sequences from long DNA molecules is very important for genome assembly and other applications. Here we describe single tube long fragment read (stLFR), a technology that enables this a low cost. It is based on adding the same barcode sequence to sub-fragments of the original long DNA molecule (DNA co-barcoding). To achieve this efficiently, stLFR uses the surface of microbeads to create million...

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Literature Corpus work: 8ff81ba3-462f-5717-bee5-1a2790cdd91f
DOI: 10.1101/324392

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