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Article

Ligation with nucleic acid sequence-based amplification.

The Journal of molecular diagnostics : JMD - 1 Jan 2000

Ong Carmichael, Tai Warren, Sarma Aartik, Opal Steven M, Artenstein Andrew W, Tripathi Anubhav

Abstract excerpt

This work presents a novel method for detecting nucleic acid targets using a ligation step along with an isothermal, exponential amplification step. We use an engineered ssDNA with two variable regions on the ends, allowing us to design the probe for optimal reaction kinetics and primer binding. This two-part probe is ligated by T4 DNA Ligase only when both parts bind adjacently to the target. The assay...

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Identifiers

PMID: 22449695
DOI: 10.1016/j.jmoldx.2012.01.004
PMCID: PMC3349837

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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