Article

Performance assessment of total RNA sequencing of human biofluids and extracellular vesicles

2019-07-14

Abstract excerpt

RNA profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite enormous interest in extracellular nucleic acids, RNA sequencing methods to quantify the total RNA content outside cells are rare. Here, we evaluate the performance of the SMARTer Stranded Total RNA-Seq method in human platelet-rich plasma, platelet-free plasma, urine, conditioned medium, and ex...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: ff1ac9ef-cb11-58b9-9d1f-b3d95f10baad
DOI: 10.1101/701524

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Performance assessment of total RNA sequencing of human biofluids and extracellular vesiclesDOI 10.1101/701524Read

Charting extracellular transcriptomes in The Human Biofluid RNA AtlasSemantic proximity Read

Depletion-assisted multiplexed cell-free RNA sequencing reveals distinct human and microbial signatures in plasma versus extracellular vesiclesSemantic proximity Read

Deep Profiling of EV Long RNAs Reveals Biofluid-Specific Transcriptomes and Splicing LandscapesSemantic proximity Read

Intact messenger RNA exists in human blood plasma and urine, and their purified macromolecular compartmentsSemantic proximity Read

Performance evaluation of RNA purification kits and blood collection tubes in the Extracellular RNA Quality Control (exRNAQC) studySemantic proximity Read

Blood collection tube and RNA purification method recommendations for extracellular RNA transcriptome profiling: A large-scale pre-analytical variable performance evaluationSemantic proximity Read

Circular RNA landscape in extracellular vesicles from human biofluidsSemantic proximity Read

Extracellular Vesicles Long RNA Sequencing Reveals Abundant mRNA, circRNA, and lncRNA in Human Blood as Potential Biomarkers for Cancer DiagnosisSemantic proximity Read

Systematic cross-study assessment of RNA-Seq experimental workflows for plasma cell-free transcriptome profilingSemantic proximity Read

Ouro-seq: Improved Recovery of Full-Length circRNAs from Samples with Limited RNA ContentSemantic proximity Read

Selective enrichment of plasma cell-free messenger RNA in cancer-associated extracellular vesiclesSemantic proximity Read

Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSCSemantic proximity Read