Article

Extended NGN2 Expression in iPSCs Dramatically Enhances Purity of Neuronal Cultures

2025-06-21

Abstract excerpt

<h4>SUMMARY</h4> iPSC-derived neuronal cultures can provide valuable insights into the pathogenesis of neurological disease. However, single-cell iPSC clones expressing NGN2 and mCherry exhibit spontaneous loss of mCherry fluorescence, raising questions about the homogeneity of neurons derived from what appear to be heterogeneous iPSCs. We find that mCherry silencing does not influence iNeurons with two lines of...

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Literature Corpus work: fda25a43-aaab-5001-aa46-238cad883c3d
DOI: 10.1101/2025.06.20.660764

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