Article

Real-Time Selective Sequencing with RUBRIC: Read Until with Basecall and Reference-Informed Criteria

2018-11-02

Abstract excerpt

The Oxford MinION, the first commercial nanopore sequencer, is also the first to implement molecule-by-molecule real-time selective sequencing or “Read Until”. As DNA transits a MinION nanopore, real-time pore current data can be accessed and analyzed to provide active feedback to that pore. Fragments of interest are sequenced by default, while DNA deemed non-informative is rejected by reversing the pore bias to e...

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Literature Corpus work: f507a506-6d9d-5f6c-8dd3-bc26b2206002
DOI: 10.1101/460014

Open publication

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