Article

Cutting Through the Artifacts: Dissecting gRNA Impurities with FUSS-seq

2025-09-17

Abstract excerpt

CRISPR-based therapeutics rely on guide RNAs (gRNAs) and the Cas9 endonuclease for precise gene editing. Ensuring gRNA purity and base-level sequence integrity is essential for clinical translation. While industry-standard practice relies on liquid chromatography-high-resolution mass spectrometry to assess oligonucleotide identity and purity, more recent FDA guidance recommends complementary base-by-base sequence...

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Literature Corpus work: b467c0ac-bc63-5d57-9a6c-d49af2e00618
DOI: 10.1101/2025.09.13.676056

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