Article

<i>In vivo</i> rescue of arboviruses directly from subgenomic DNA fragments

2024-01-20

Abstract excerpt

Reverse genetic systems are mainly used to study RNA viruses and rescue recombinant strains in cell culture. Here, we provide proof-of-concept for direct in vivo viral generation using the ‘Infectious Subgenomic Amplicons’ method. So far, this procedure allowed to rescue in vitro RNA viruses, by the transfection of several overlapping subgenomic DNA fragments encoding the entire virus genome. We adapted and opt...

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Literature Corpus work: f45c1273-6a1a-5645-bf16-775f05f38b70
DOI: 10.1101/2024.01.17.576038

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