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SuPReMe: a rapid reverse genetics method to generate clonal populations of recombinant RNA viruses

21 Mar 2018

Abstract excerpt

Reverse genetics systems enable the manipulation of viral genomes and are proving to be essential for studying RNA viruses. Methods for generating clonal virus populations are particularly useful for studying the impact of genomic modifications on viral properties. Here, by exploiting a chikungunya virus model, we compare viral populations and their replicative fitness when generated using either the rapid and...

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PMID: 29559627
DOI: 10.1038/s41426-018-0040-2

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