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Article

Comparison of chikungunya viruses generated using infectious clone or the Infectious Subgenomic Amplicons (ISA) method in Aedes mosquitoes.

PloS one - 1 Jan 2018

Mohamed Ali Souand, Vega-Rúa Anubis, Driouich Jean-Sélim, de Lamballerie Xavier, Failloux Anna-Bella, Nougairède Antoine

Abstract excerpt

Reverse genetics systems provide the opportunity to manipulate viral genomes and have been widely used to study RNA viruses and to develop new antiviral compounds and vaccine strategies. The recently described method called ISA (Infectious Subgenomic Amplicons) gives the possibility to rescue RNA viruses in days. We demonstrated in cell culture that the use of the ISA method led to a higher genetic diversity of...

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Identifiers

PMID: 29953474
DOI: 10.1371/journal.pone.0199494
PMCID: PMC6023229

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