Article

A 3D human liver model of nonalcoholic steatohepatitis

2020-02-07

Abstract excerpt

<h4> A bstract </h4> We have developed an in vitro preclinical 3D Non-Alcoholic SteatoHepatitis (NASH) model by co-culturing four human primary liver cell types: hepatocytes, stellate, endothelial and Kupffer cells. Cells were embedded in a hydrogel of rat collagen in 96-well plate and a NASH-like environment was induced with a medium containing free fatty acids (FFAs) and tumor necrosis factor α (TNFα). This...

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Literature Corpus work: f23eca02-ded0-5256-85cc-cf1e0502778f
DOI: 10.1101/2020.02.07.938787

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