Article

A high-throughput methods SNIPR-Nm reveals small RNA methylation ratio

2025-12-13

Abstract excerpt

Small RNAs regulate gene expression and genome stability, and their functions are shaped by 3′-terminal 2′-O-methylation (Nm). Although Nm is universal in plants and mainly marks animal piRNAs, recent evidence reveals Nm on mammalian miRNAs, highlighting broader regulatory roles. However, current sequencing underestimates Nm-modified RNAs due to ligation bias, and existing qPCR-based methods cannot simultaneously...

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Literature Corpus work: f1e0a45c-78dc-5041-b287-cccc500d10a9
DOI: 10.64898/2025.12.11.693694

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