Article

Antibody-free enzyme-assisted chemical labeling for detection of transcriptome-wide <i>N</i> <sup>6</sup> -methyladenosine

2019-10-02

Abstract excerpt

The inert chemical property of RNA modification N 6 -methyladenosine (m 6 A) makes it very challenging to detect, and all of the transcriptome-wide m 6 A detection methods rely on m 6 A-antibody immunoprecipitation. However, their results are dependent on the quality and specificity of antibodies. Although the endoribonuclease-based single-base m 6 A sequencing is antibody-free, it maps only 16~25% sites. Her...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: aff8db37-c41e-530b-98c8-f898736f18a0
DOI: 10.1101/790683

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Antibody-free enzyme-assisted chemical labeling for detection of transcriptome-wide <i>N</i> <sup>6</sup> -methyladenosineDOI 10.1101/790683Read

Single-base mapping of m <sup>6</sup> A by an antibody-independent methodSemantic proximity Read

Advanced eTAM-seq enables high-fidelity, low-input <i>N</i> <sup>6</sup> -methyladenosine profiling in human cells and embryonic mouse tissuesSemantic proximity Read

i <sup>6</sup> A-seq maps <i>N</i> <sup>6</sup> -isopentenyladenosine and uncovers its role as a regulator of mRNA stability through recruitment of DIS3L2Semantic proximity Read

Identification of differentially methylated single-nucleotide m <sup>6</sup> A sites by incorporating site-specific antibody specificitySemantic proximity Read

Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome.Semantic proximity Read

Multiplexed In Situ Imaging of Site-Specific m6A Methylation with Proximity Hybridization Followed by Primer Exchange Amplification (m6A-PHPEA)Semantic proximity Read

Transcriptome-wide identification of 5-methylcytosine by deaminase and reader protein-assisted sequencingSemantic proximity Read

Landscape and regulation of m <sup>6</sup> A and m <sup>6</sup> Am methylome across human and mouse tissuesSemantic proximity Read

Bisulfite-free and single-nucleotide resolution sequencing of DNA epigenetic modification of 5-hydroxymethylcytosine using engineered deaminaseSemantic proximity Read

Comprehensive analysis across mammalian tissues and cells decipher the underlying mechanism of m6A specificitySemantic proximity Read

m5C-Atlas: a comprehensive database for decoding and annotating the 5-methylcytosine (m5C) epitranscriptome.Semantic proximity Read

Landscape and Regulation of m6A and m6Am Methylome across Human and Mouse Tissues.Semantic proximity Read