Article

DSB repair kinetics impair CRISPR-Cas9 editing in human embryos

2025-05-10

Abstract excerpt

Cas9 provides a powerful tool to interrogate DNA repair and to introduce targeted genetic modifications. However, a major challenge of Cas9-based editing in human embryos is the occurrence of chromosomal abnormalities caused by Cas9 cleavage. Furthermore, mosaicism - different genetic outcomes in different cells, prevent accurate genotyping using a single embryo biopsy. Through timed analysis of editing outcomes d...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: eeae3746-5886-5ff4-a8c5-f5a5c6fa4eb7
DOI: 10.1101/2025.05.07.652614

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

DSB repair kinetics impair CRISPR-Cas9 editing in human embryosDOI 10.1101/2025.05.07.652614Read

Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos.Semantic proximity Read

DNA Double Strand Breaks cause chromosome loss through sister chromatid tethering in human embryosSemantic proximity Read

The Human Cleavage Stage Embryo Is a Cradle of Chromosomal RearrangementsSemantic proximity Read

Efficient base editing and development in human embryos without chromosomal alterationsSemantic proximity Read

Reading frame restoration at the EYS locus, and allele-specific chromosome removal after Cas9 cleavage in human embryosSemantic proximity Read

Tripolar mitosis and partitioning of the genome arrests human preimplantation development in vitro.Semantic proximity Read

Sperm DNA damage causes genomic instability in early embryonic developmentSemantic proximity Read

Retained chromosomal integrity following CRISPR-Cas9-based mutational correction in human embryos.Semantic proximity Read

Single-Cell Sequencing of Primate Preimplantation Embryos Reveals Chromosome Elimination Via Cellular Fragmentation and Blastomere ExclusionSemantic proximity Read

Cas9 in Human Embryos: On Target but No Repair.Semantic proximity Read

Molecular contribution to embryonic aneuploidy and karyotypic complexity in initial cleavage divisions of mammalian developmentSemantic proximity Read

Correction of a pathogenic gene mutation in human embryos.Semantic proximity Read