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Article

BMP4-induced symmetry breaking in a 3D model of the human epiblast

2019-11-20

Abstract excerpt

<title>Abstract</title> <p>We describe the protocol of generating a 3D stem-cell-based model of the human pre-gastrulation epiblast by culturing human embryonic stem cells in a mix of hydrogel and Matrigel. Much like the epiblast of an <italic>in vitro</italic> attached day-10 human embryo, this model is an epithelial sphere with a cavity at its center, it is expressing key pluripotency markers, and it displays a...

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Literature Corpus work
ee8facb2-bfa0-5579-a157-513730d42d9e
DOI
10.21203/rs.2.9730/v1
Open publication

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BMP4-induced symmetry breaking in a 3D model of the human epiblastDOI 10.21203/rs.2.9730/v1
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